Manufactured by:BT LAB
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Human Long-chain Fatty Acid Transport Protein 4, SLC27A4 ELISA Kit

Human Long-chain Fatty Acid Transport Protein 4, SLC27A4 ELISA Kit

SKU:
ABM216916
Manufacturer SKU
E5594Hu
Product type
ELISA Kits
TargetSLC27A4
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Involved in translocation of long-chain fatty acids (LFCA) across the plasma membrane (PubMed: 12556534, PubMed: 21395585). Has acyl-CoA ligase activity for long-chain and very-long-chain fatty acids (VLCFAs) (PubMed: 24269233). Appears to be the principal fatty acid transporter in small intestinal enterocytes. Plays a role in the formation of the epidermal barrier. Required for fat absorption in early embryogenesis (By similarity). Probably involved in fatty acid transport across the blood barrier (PubMed: 21395585). Indirectly inhibits RPE65 via substrate competition and via production of VLCFA derivatives like lignoceroyl-CoA. Prevents light-induced degeneration of rods and cones (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SLC27A4 antibody. SLC27A4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SLC27A4 Antibody is added and binds to SLC27A4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SLC27A4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SLC27A4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Neuroscience
Also known as
ACSVL4;Arachidonate--CoA ligase;FATP-4;Fatty acid transport protein 4;Long-chain fatty acid transport protein 4;Long-chain-fatty-acid--CoA ligase;SLC27A4;Solute carrier family 27 member 4;Very long-chain acyl-CoA synthetase 4;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
23.42ng/L
Detection range
50-10000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
72,064 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5594Hu
Gene symbol
SLC27A4
Gene ID
10999
UniProt accession
Q6P1M0
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00