Manufactured by:BT LAB
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Human Glutathione Synthetase, GSS ELISA Kit

Human Glutathione Synthetase, GSS ELISA Kit

SKU:
ABM216522
Manufacturer SKU
E5347Hu
Product type
ELISA Kits
TargetGSS
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Glutathione synthetase (GSS) (EC 6.3.2.3) is the second enzyme in the glutathione (GSH) biosynthesis pathway. It catalyses the condensation of gamma-glutamylcysteine and glycine, to form glutathione. Glutathione synthetase is also a potent antioxidant. It is found in many species including bacteria, yeast, mammals, and plants. In humans, defects in GSS are inherited in an autosomal recessive way and are the cause of severe metabolic acidosis, 5-oxoprolinuria, increased rate of haemolysis, and defective function of the central nervous system. Deficiencies in GSS can cause a spectrum of deleterious symptoms in plants and human beings alike.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human GSS antibody. GSS present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GSS Antibody is added and binds to GSS in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GSS antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GSS. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction, Neuroscience, Cell Biology, Metabolism
Also known as
Glutathione Synthase;Glutathione synthetase;GSH synthetase;GSHS;GSH-S;GSS;HEL-S-64p;HEL-S-88n
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.028U/mL
Detection range
0.05-20U/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
52,385 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5347Hu
Gene symbol
GSS
Gene ID
2937
UniProt accession
P48637
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

GSSglutathione synthetase

View target guide

GSS identifies glutathione synthetase in Homo sapiens (Human). This target record connects NCBI Gene 2937, UniProtKB P48637 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
2937
UniProtKB
P48637
BT LAB
£240.93£205.00