Mouse Heat Shock Protein Beta-7, HSPB7 ELISA Kit

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Mouse Heat Shock Protein Beta-7, HSPB7 ELISA Kit

Mouse Heat Shock Protein Beta-7, HSPB7 ELISA Kit

SKU:
ABM211862
Manufacturer SKU
E2123Mo
Product type
ELISA Kits
TargetHSPB7
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Heat Shock Protein Family B (small) member 7 (HSPB7) in humans is a protein encoded by a gene of the same name with four exons that is located on chromosome 1p36.13. HSPB7 contains 170 amino acids and has a molecular weight of 18,611Da. HSPB7 is a member of human small heat shock protein (HSPB) family, which contains eleven family members of chaperone proteins.[7] HSPB7 and its gene pair SRARP are located 5 kb apart on the opposite strands of chromosome 1p36.13.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse HSPB7 antibody. HSPB7 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse HSPB7 Antibody is added and binds to HSPB7 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HSPB7 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse HSPB7. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
27kDa;Cardiovascular heat shock protein;cv;cvHsp;Heat shock protein 25 kDa 2;Heat shock protein beta-7;Hsp25;Hsp25-2;HSPB7;Protein p19/6.8
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
5.21ng/L
Detection range
10-2000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
18,635 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2123Mo
Gene symbol
Hspb7
Gene ID
29818
UniProt accession
P35385
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00