Manufactured by:BT LAB
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Rat Purkinje Cell Protein 4, PCP4 ELISA Kit

Rat Purkinje Cell Protein 4, PCP4 ELISA Kit

SKU:
ABM210710
Manufacturer SKU
E1709Ra
Product type
ELISA Kits
TargetPCP4
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue, Cell culture supernatants, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Functions as a modulator of calcium-binding by calmodulin. Thereby, regulates calmodulin activity and the different processes it controls. For instance, may play a role in neuronal differentiation through activation of calmodulin-dependent kinase signaling pathways.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat PCP4 antibody. PCP4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat PCP4 Antibody is added and binds to PCP4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PCP4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat PCP4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction, Neuroscience
Also known as
Pcp4;Pep19
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.11ng/ml
Detection range
0.19-12ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
6,807 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaUrineAscitesCerebrospinal fluidTissueCell culture supernatantsTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1709Ra
Gene symbol
Pcp4
Gene ID
25510
UniProt accession
P63055
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8°C
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00