Porcine IL-20 (Interleukin 20) Fast Step ELISA Kit

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine IL-20. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Porcine IL-20 are added to the micro ELISA plate wells. Porcine IL-20 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Porcine IL-20. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Porcine IL-20. You can calculate the concentration of Porcine IL-20 in the samples by comparing the OD of the samples to the standard curve.