Manufactured by:ELK Biotechnology
RUO

FGF-23 rabbit pAb

FGF-23 rabbit pAb from ELK Biotechnology

SKU:
ABM109401
Product type
Primary Antibody
TargetFGF-23
ApplicationsWB, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemExpressed in osteogenic cells particularly during phases of active bone remodeling. In adult trabecular bone, expressed in osteocytes and flattened bone-lining cells (inactive osteoblasts).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

FGF-23 rabbit pAb is a research-use antibody from ELK Biotechnology for studying FGF-23 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR). Mutations in this gene are also associated with hyperphosphatemic familial tumoral calcinosis (HFTC). [provided by RefSeq, Feb 2013],

Additional antibody information

Specificity FGF-23 Polyclonal Antibody detects endogenous levels of FGF-23 protein.
Validation evidence captions Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min. | Immunofluorescence analysis of HUVEC cells, using FGF23 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
>>MAPK signaling pathway, >>Ras signaling pathway, >>Rap1 signaling pathway, >>Calcium signaling pathway, >>PI3K-Akt signaling pathway, >>Regulation of actin cytoskeleton, >>Parathyroid hormone synthesis, secretion and action, >>Pathways in cancer, >>Melanoma, >>Breast cancer, >>Gastric cancer
Also known as
FGF23; HYPF; Fibroblast growth factor 23; FGF-23; Phosphatonin; Tumor-derived hypophosphatemia-inducing factor
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human FGF23. AA range:151-200
Observed band
27kD
Cellular localization
Secreted . Secretion is dependent on O-glycosylation.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 151-200
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES7620
UniProt entry
Q9GZV9
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00