Manufactured by:ELK Biotechnology
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NRF-1 rabbit pAb

NRF-1 rabbit pAb from ELK Biotechnology

SKU:
ABM108973
Manufacturer SKU
ES6416
Product type
Primary Antibodies
TargetNRF-1
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat, Bovine
Expression systemUbiquitously expressed with strongest expression in skeletal muscle.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

NRF-1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying NRF-1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat, Bovine. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014],

Additional antibody information

Specificity NRF-1 Polyclonal Antibody detects endogenous levels of NRF-1 protein.
Validation evidence captions Western Blot analysis of 3T3 cells using NRF-1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). | Western blot analysis of NRF1 Antibody. The lane on the right is blocked with the NRF1 peptide. | Western blot analysis of the lysates from HeLa cells using NRF1 antibody.
Key facts & specifications
Research areas
Apelin signaling pathway, Huntington disease
Also known as
NRF1; Nuclear respiratory factor 1; NRF-1; Alpha palindromic-binding protein; Alpha-pal
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human NRF1. AA range:221-270
Observed band
54kD
Cellular localization
Nucleus.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 221-270
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Bovine
Identifiers & database references
Manufacturer SKU
ES6416
UniProt entry
Q16656
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00£176.00