Manufactured by:ELK Biotechnology
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Integrin β3 rabbit pAb

Integrin β3 rabbit pAb from ELK Biotechnology

SKU:
ABM107184
Manufacturer SKU
ES2630
Product type
Primary Antibodies
TargetIntegrin β3
ApplicationsWB, IHC, IF/ICC, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemIsoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Integrin β3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Integrin β3 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Integrin β3 Polyclonal Antibody detects endogenous levels of Integrin β3 protein.
Validation evidence captions Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min. | Western Blot analysis of various cells using Integrin β3 Polyclonal Antibody diluted at 1:500 | Western Blot analysis of NIH-3T3 cells using Integrin β3 Polyclonal Antibody diluted at 1:500 | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Integrin beta3 Antibody. The picture on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
Rap1 signaling pathway, Phagosome, PI3K-Akt signaling pathway, Osteoclast differentiation, Focal adhesion, ECM-receptor interaction, Platelet activation, Neutrophil extracellular trap formation, Hematopoietic cell lineage, Regulation of actin cytoskeleton, Thyroid hormone signaling pathway, Human cytomegalovirus infection, Human papillomavirus infection, Herpes simplex virus 1 infection, Proteoglycans in cancer, MicroRNAs in cancer, Hypertrophic cardiomyopathy, Arrhythmogenic right ventricular cardiomyopathy, Dilated cardiomyopathy, Fluid shear stress and atherosclerosis
Also known as
ITGB3; GP3A; Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD antigen CD61
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human Integrin beta3. AA range:739-788
Observed band
110kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein . Cell projection, lamellipodium membrane . Cell junction, focal adhesion . Cell junction, synapse, postsynaptic cell membrane ; Single-pass type I membrane protein . Cell junction, synapse .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 739-788
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Manufacturer SKU
ES2630
UniProt entry
P05106
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00£176.00