Manufactured by:ELK Biotechnology
Save 20%RUO

EF-2 rabbit pAb

EF-2 rabbit pAb from ELK Biotechnology

SKU:
ABM107009
Manufacturer SKU
ES2230
Product type
Primary Antibodies
TargeteEF2
ApplicationsWB, IHC, IF/ICC, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemBrain,Cajal-Retzius cell,Epithelium,Hepatocyte,Ovary,Periph
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

EF-2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying eEF2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity EF-2 Polyclonal Antibody detects endogenous levels of EF-2 protein.
Validation evidence captions Western Blot analysis of various cells using EF-2 Polyclonal Antibody diluted at 1:2000 | Western Blot analysis of VEC cells using EF-2 Polyclonal Antibody diluted at 1:2000 | Immunofluorescence analysis of HUVEC cells, using eEF2 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using eEF2 Antibody. The picture on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
AMPK signaling pathway, Oxytocin signaling pathway
Also known as
EEF2; EF2; Elongation factor 2; EF-2
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human eEF2. AA range:31-80
Observed band
100kD
Cellular localization
Cytoplasm . Nucleus . Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 31-80
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Manufacturer SKU
ES2230
UniProt entry
P13639
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

EEF2eukaryotic translation elongation factor 2

View target guide

EEF2 identifies eukaryotic translation elongation factor 2 in Homo sapiens (Human). This target record connects NCBI Gene 1938, UniProtKB P13639 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
1938
UniProtKB
P13639
ELK Biotechnology
£220.00£176.00