Manufactured by:ELK Biotechnology
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EAAT3 rabbit pAb

EAAT3 rabbit pAb from ELK Biotechnology

SKU:
ABM107003
Manufacturer SKU
ES2217
Product type
Primary Antibodies
TargetEAAT3
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemExpressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

EAAT3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying EAAT3 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are thought to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect. [provided by RefSeq, Mar 2010],

Additional antibody information

Specificity EAAT3 Polyclonal Antibody detects endogenous levels of EAAT3 protein.
Validation evidence captions Western Blot analysis of various cells using EAAT3 Polyclonal Antibody | Western Blot analysis of 293 cells using EAAT3 Polyclonal Antibody | Western blot analysis of lysate from 293 cells treated with EGF, using EAAT3 antibody.
Key facts & specifications
Research areas
Synaptic vesicle cycle, Glutamatergic synapse, Protein digestion and absorption
Also known as
SLC1A1; EAAC1; EAAT3; Excitatory amino acid transporter 3; Excitatory amino-acid carrier 1; Neuronal and epithelial glutamate transporter; Sodium-dependent glutamate/aspartate transporter 3; Solute carrier family 1 member 1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human EAAT3. AA range:122-171
Observed band
57kD
Cellular localization
Cell membrane ; Multi-pass membrane protein . Apical cell membrane ; Multi-pass membrane protein . Cell junction, synapse, synaptosome . Early endosome membrane . Late endosome membrane . Recycling endosome membrane .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 122-171
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Manufacturer SKU
ES2217
UniProt entry
P43005
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SLC1A1solute carrier family 1 member 1

View target guide

SLC1A1 identifies solute carrier family 1 member 1 in Homo sapiens (Human). This target record connects NCBI Gene 6505, UniProtKB P43005 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6505
UniProtKB
P43005
ELK Biotechnology
£220.00£176.00