Human ATG16L1(Autophagy Related Protein 16 Like Protein 1) ELISA Kit from ELK Biotechnology
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Human ATG16L1(Autophagy Related Protein 16 Like Protein 1) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring ATG16L1 in Tissue homogenates, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ATG16L1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ATG16L1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ATG16L1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ATG16L1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include WDR30.
Fully tested in-house by ABMIUM, or supported by an advanced validation report from a partner reviewed by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Sample compatibility evidence using the same validation key.
| Sample type | Tissue homogenates | Other biological fluids |
|---|---|---|
| Sample compatibility |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
ATG16L1 encodes an autophagy factor that works with the ATG12-ATG5 complex to drive ATG8-family protein lipidation in canonical and non-canonical autophagy. This target record connects reviewed human ATG16L1 identifiers with ABMIUM catalogue products targeting ATG16L1.
Biological function: ATG16L1 is an essential component of canonical and non-canonical autophagy. Together with ATG12-ATG5, it forms an E3-like machinery that promotes phosphatidylethanolamine conjugation of ATG8-family proteins such as LC3 and GABARAP family members. ATG16L1 also acts as a molecular hub that coordinates distinct autophagy pathways and contributes to antibacterial autophagy.
