Manufactured by:ELK Biotechnology
RUO

Histone H3(Phospho Thr32 ) Rabbit pAb

Histone H3(Phospho Thr32 ) Rabbit pAb from ELK Biotechnology

SKU:
ABM102029
Manufacturer SKU
EA064
Product type
Primary Antibodies
TargetHistone H3(Phospho Thr32 )
ApplicationsWB, IHC
Species / reactivityHuman, Rat, Mouse

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Histone H3(Phospho Thr32 ) Rabbit pAb is a research-use antibody from ELK Biotechnology for studying Histone H3(Phospho Thr32 ) in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Additional antibody information

Validation evidence captions Western blot analysis of extracts from Hela cells, untreated (-) or treated with Nocodazole (1µg/ml, 24 hr; +), using Phospho-Histone H3 (Thr32) Rabbit pAb (upper) EA064 1:2000 and Histone H3 (1G1) Mouse mAb (lower) EM1037 1:2000. | Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using Histone H3(Phospho Thr32)(EA064)Rabbit pAb diluted at 1:500.
Key facts & specifications
Research areas
Antibody Research
Also known as
H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H7a antibody
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:1,000-2,000 IHC 1:200-500
Immunogen
Synthetic Peptide
Observed band
15-17kDa
Cellular localization
Nuclear
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:200-500
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Manufacturer SKU
EA064
UniProt entry
P68431
Storage, stability & shipping
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage warning
Avoid repeated freeze-thaw cycles.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£176.00