Manufactured by:Boster Bio
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Anti-ATP5F1,2,3/ATP5MC1,2,3 Picoband® Antibody (monoclonal, 12E9

Primary Antibody supplied by Boster Bio

SKU:
ABM23578-1
Manufacturer SKU
M09735
Product type
Primary Antibodies
TargetATP synthase F
ApplicationsELISA, Flow Cytometry, IHC, IHC-P, WB
Species / reactivityHuman, Mouse, Rat, Monkey

Manufacturer provenance

Manufactured by:
Boster Bio
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Anti-ATP5F1,2,3/ATP5MC1,2,3 Picoband® Antibody (monoclonal, 12E9 is a research-use antibody from Boster Bio for studying ATP synthase F in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-ATP5F1,2,3/ATP5MC1,2,3 Picoband® Antibody (monoclonal, 12E9) catalog # M09735. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

The ATP5MC1 gene is one of three human paralogs that encode membrane subunit c of the mitochondrial ATP synthase. It is mapped to 17q21.32. This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified.

Product information

Key facts & specifications
Also known as
ATP5MC1; ATP5G1; ATPase protein 9; ATPase subunit c; ATP5A; ATP5G
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: 12E9
Isotype
Mouse IgG2b
Cross-reactivity
No cross-reactivity with other proteins.
Dilution range
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat, Monkey
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human, Mouse, Rat
Immunogen
E.coli-derived human ATP5G1,2,3/ATP5MC1,2,3 recombinant protein (Position: D62-L113).
Observed band
10-14 kDa
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification
Immunogen affinity purified.
Form / buffer
Lyophilized
Sequence fragment
E.coli-derived human ATP5G1,2,3/ATP5MC1,2,3 recombinant protein (Position: D62-L113).
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Mouse
Rat
Monkey
Identifiers & database references
Manufacturer SKU
M09735
Gene symbol
ATP5MC1,2,3
Gene ID
516
UniProt accession
P05496
Storage, stability & shipping
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Storage warning
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Compliance & documents
CE marked
No
Datasheet
Open datasheet
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Boster Bio and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Boster Bio
£328.00£278.80