Manufactured by:ELK Biotechnology
Save 20%RUO

53BP1 (phospho-Ser1778) rabbit pAb

53BP1 (phospho-Ser1778) rabbit pAb from ELK Biotechnology

SKU:
ABM106718
Manufacturer SKU
ES18530
Product type
Primary Antibodies
Target53BP1
ApplicationsWB
Species / reactivityHuman, Rat, Mouse
Expression systemCerebellum,Cervix,Epithelium,Myeloid leukemia cell,Skeletal muscle,
PurityThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

53BP1 (phospho-Ser1778) rabbit pAb is a research-use antibody from ELK Biotechnology for studying 53BP1 in WB workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of Human 53BP1 (phospho-Ser1778)
Validation evidence captions Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
Key facts & specifications
Research areas
NOD-like receptor signaling pathway
Also known as
Tumor suppressor p53-binding protein 1 (53BP1) (p53-binding protein 1) (p53BP1)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:1000-2000
Immunogen
Synthesized phosho peptide around human 53BP1 (Ser1778)
Observed band
213kD
Cellular localization
Nucleus . Chromosome . Chromosome, centromere, kinetochore . Localizes to the nucleus in absence of DNA damage (PubMed:28241136). Following DNA damage, recruited to sites of DNA damage, such as double stand breaks (DSBs): recognizes and binds histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23333306, PubMed:23760478, PubMed:24703952, PubMed:28241136, PubMed:17190600). Associated with kinetochores during mitosis (By similarity). .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Manufacturer SKU
ES18530
UniProt entry
Q12888
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

TP53BP1tumor protein p53 binding protein 1

View target guide

TP53BP1 identifies tumor protein p53 binding protein 1 in Homo sapiens (Human). This target record connects NCBI Gene 7158, UniProtKB Q12888 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
7158
UniProtKB
Q12888
ELK Biotechnology
£220.00£176.00