Rat ASMA(Anti-Smooth Muscle Antibody) ELISA Kit from ELK Biotechnology
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Rat ASMA(Anti-Smooth Muscle Antibody) ELISA Kit is a research-use antibody from ELK Biotechnology for studying ASMA in ELISA workflows with listed reactivity for Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ASMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ASMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ASMA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ASMA in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include ASM Ab; Anti Smooth Muscle Antibody.
Fully tested in-house by ABMIUM, or supported by an advanced validation report from a partner reviewed by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Sample compatibility evidence using the same validation key.
| Sample type | Serum | Plasma | Tissue homogenates | Other biological fluids |
|---|---|---|---|---|
| Sample compatibility |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
Actin alpha 1, skeletal muscle (ACTA1) encodes the skeletal-muscle alpha-actin protein, also named actin, alpha skeletal muscle and alpha-actin-1. Human ACTA1 maps to NCBI Gene 58 and reviewed UniProtKB P68133; this target record links the verified target to related ABMIUM catalogue products.
Biological function: ACTA1 is an alpha-actin isoform of skeletal muscle and a major constituent of the contractile apparatus. Actins are highly conserved proteins involved in cell structure and motility; reviewed UniProt additionally associates ACTA1 with muscle contraction, skeletal-muscle fibre development and thin-filament assembly.
