Recombinant Mouse IFN- Lambda2/IL-28A protein

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Recombinant Mouse IFN- Lambda2/IL-28A protein

Recombinant Mouse IFN- Lambda2/IL-28A protein

SKU:
ABM219670
Manufacturer SKU
CD00408
Product type
Recombinant Proteins
TargetRecombinant Mouse IFN- Lambda2/IL-28A protein
Expression systemEscherichia coli.
Purity> 95 % by SDS-PAGE and HPLC analyses.

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Mouse IFN-lambda 2 (IL-28A), IFN-lambda 3 (IL-28B) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid sequence identity) and type I IFN family (15 - 19% a.a. sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28A shares 64%, 65%, and 56% a.a. identity with human IL-28A, IL-28B, and IL-29, respectively.

Expression system
Escherichia coli.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
Molecular weight
Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.
Endotoxin
Less than 0.1 EU/μg of rMuIFN-λ2/IL-28A as determined by LAL method.
Form
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % trehalose.

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Key facts & specifications
Research areas
Immunology
Also known as
IFN-λ2/IL-28A, Il; Il28a; IL-28A; If1ia1; EG330496,Ifnl2,Mouse
Source organism
Mouse
Molecular weight
Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.
Form / buffer
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % trehalose.
Activity
Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 105 IU/mg.
Endotoxin level
Less than 0.1 EU/μg of rMuIFN-λ2/IL-28A as determined by LAL method.
Sequence
DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKDAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENMTDS ALATILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFRLL TRDLKCVASG DQCV
Quantity per pack
5 ug
Technical note
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSDS-PAGEHPLCLALBioactivity
Quality control
Identifiers & database references
Manufacturer SKU
CD00408
Storage, stability & shipping
Storage conditions
-20ºC
Storage buffer
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % trehalose.
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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