Manufactured by:BT LAB
Save 15%RUO

Recombinant Rat IL-1 Alpha protein

Recombinant Rat IL-1 Alpha protein

SKU:
ABM219134
Manufacturer SKU
CD00114
Product type
Recombinant Proteins
TargetRecombinant Rat IL-1 Alpha protein
Expression systemEscherichia coli.
Purity> 97 % by SDS-PAGE and HPLC analyses.

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Interleukin-1 alpha (IL-1α) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties. Recombinant rat IL-1 alpha contains 150 amino acids which is a single non-glycosylated polypeptide.

Expression system
Escherichia coli.
Purity
> 97 % by SDS-PAGE and HPLC analyses.
Molecular weight
Approximately 17.8 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.
Endotoxin
Less than 1 EU/μg of rRtIL-1α as determined by LAL method.
Form
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 150 mM NaCl, pH 8.5.

Packaging image shown for reference. Select a size from the available options.

Key facts & specifications
Research areas
Immunology
Also known as
IL-1α,IL-1 alpha, IL-1F1,IL1A, Rat
Source organism
Rat
Molecular weight
Approximately 17.8 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.
Form / buffer
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 150 mM NaCl, pH 8.5.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse D10S cells is less than 0.005 ng/ml, corresponding to a specific activity of > 2.0 × 108 IU/mg.
Endotoxin level
Less than 1 EU/μg of rRtIL-1α as determined by LAL method.
Sequence
SAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDDSKYP VTLKVSNTQL FVSAQGEDKP VLLKEIPETP KLITGSETDL IFFWEKINSK NYFTSAAFPE LLIATKEQSQ VHLARGLPSM IDFQIS
Quantity per pack
10 ug, 2 ug
Technical note
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSDS-PAGEHPLCLALBioactivity
Quality control
Identifiers & database references
Manufacturer SKU
CD00114
UniProt accession
P16599
Storage, stability & shipping
Storage conditions
-20ºC
Storage buffer
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 150 mM NaCl, pH 8.5.
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£307.94£262.00