Manufactured by:BT LAB
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Human Beta-galactoside Alpha-2, ST6GAL1 ELISA Kit

Human Beta-galactoside Alpha-2, ST6GAL1 ELISA Kit

SKU:
ABM218304
Manufacturer SKU
E6553Hu
Product type
ELISA Kits
TargetST6GAL1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human ST6GAL1 antibody. ST6GAL1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ST6GAL1 Antibody is added and binds to ST6GAL1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ST6GAL1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ST6GAL1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology, Signal Transduction
Also known as
Alpha 2,6-ST 1;B-cell antigen CD75;Beta-galactoside alpha-2,6-sialyltransferase 1;CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1;Sialyltransferase 1;SIAT1;ST6Gal I;ST6GAL1;ST6GalI;ST6N
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.48ng/mL
Detection range
1-400ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
46,605 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E6553Hu
Gene symbol
ST6GAL1
Gene ID
6480
UniProt accession
P15907
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ST6GAL1ST6 beta-galactoside alpha-2,6-sialyltransferase 1

View target guide

ST6GAL1 identifies ST6 beta-galactoside alpha-2,6-sialyltransferase 1 in Homo sapiens (Human). This target record connects NCBI Gene 6480, UniProtKB P15907 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6480
UniProtKB
P15907
BT LAB
£240.93£205.00