Human Zinc Finger Protein Gli1, GLI1 ELISA Kit

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Human Zinc Finger Protein Gli1, GLI1 ELISA Kit

Human Zinc Finger Protein Gli1, GLI1 ELISA Kit

SKU:
ABM218144
Manufacturer SKU
E6417Hu
Product type
ELISA Kits
TargetGLI1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Acts as a transcriptional activator (PubMed: 19706761, PubMed: 10806483, PubMed: 19878745, PubMed: 24311597, PubMed: 24217340). Binds to the DNA consensus sequence 5'-GACCACCCA-3' (PubMed: 2105456, PubMed: 8378770, PubMed: 24217340). Regulates the transcription of specific genes during normal development (PubMed: 19706761). Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed: 19706761, PubMed: 28973407). Plays a role in cell proliferation and differentiation via its role in SHH signaling (PubMed: 11238441, PubMed: 28973407). [Isoform 2]: Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human GLI1 antibody. GLI1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GLI1 Antibody is added and binds to GLI1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GLI1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GLI1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
GLI 1;GLI1;Glioma-associated oncogene;Oncogene GLI;Zinc finger protein GLI1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
7.85ng/L
Detection range
15-3000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
117,904 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E6417Hu
Gene symbol
GLI1
Gene ID
2735
UniProt accession
P08151
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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