Manufactured by:BT LAB
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Human Suppressor of Tumorigenicity 14 Protein, ST14 ELISA Kit

Human Suppressor of Tumorigenicity 14 Protein, ST14 ELISA Kit

SKU:
ABM217852
Manufacturer SKU
E6235Hu
Product type
ELISA Kits
TargetST14
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Degrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site. Involved in the terminal differentiation of keratinocytes through prostasin (PRSS8) activation and filaggrin (FLG) processing.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human ST14 antibody. ST14 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ST14 Antibody is added and binds to ST14 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ST14 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ST14. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
ARCI11;CAP3;HAI;Matriptase;Membrane-type serine protease 1;MTSP1;MT-SP1;Prostamin;Serine protease 14;Serine protease TADG-15;SNC19;ST14;Suppressor of tumorigenicity 14 protein;TADG15;TMPRSS14;Tumor-associated differentially-expressed gene 15 protein
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.032ng/mL
Detection range
0.05-20ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
94,770 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E6235Hu
Gene symbol
ST14
Gene ID
6768
UniProt accession
Q9Y5Y6
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ST14ST14 transmembrane serine protease matriptase

View target guide

ST14 identifies ST14 transmembrane serine protease matriptase in Homo sapiens (Human). This target record connects NCBI Gene 6768, UniProtKB Q9Y5Y6 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6768
UniProtKB
Q9Y5Y6
BT LAB
£240.93£205.00