Manufactured by:BT LAB
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Human Low Affinity Immunoglobulin Gamma Fc Region Receptor 3-B, FCGR3B ELISA Kit

Human Low Affinity Immunoglobulin Gamma Fc Region Receptor 3-B, FCGR3B ELISA Kit

SKU:
ABM216922
Manufacturer SKU
E5598Hu
Product type
ELISA Kits
TargetFCGR3B
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human FCGR3B antibody. FCGR3B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FCGR3B Antibody is added and binds to FCGR3B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FCGR3B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human FCGR3B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
CD antigen CD16b;CD16;CD16A;CD16b;FCG3;Fc-gamma RIII;Fc-gamma RIIIb;Fc-gamma RIII-beta;FCGR3;FCGR3A;FCGR3B;FcR10;FcR-10;FCRIII;FCRIIIb;IGFR3;IgG Fc receptor III-1;Low affinity immunoglobulin gamma Fc region receptor III-B
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.29ng/mL
Detection range
0.5-200ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
26,216 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5598Hu
Gene symbol
FCGR3B
Gene ID
2215
UniProt accession
O75015
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

FCGR3BFc gamma receptor IIIb

View target guide

FCGR3B identifies Fc gamma receptor IIIb in Homo sapiens (Human). This target record connects NCBI Gene 2215, UniProtKB O75015 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
2215
UniProtKB
O75015
BT LAB
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