Manufactured by:BT LAB
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Human E3 Ubiquitin-protein Ligase Mdm2, MDM2 ELISA Kit

Human E3 Ubiquitin-protein Ligase Mdm2, MDM2 ELISA Kit

SKU:
ABM216298
Manufacturer SKU
E5213Hu
Product type
ELISA Kits
TargetMDM2
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Mouse double minute 2 homolog (MDM2) also known as E3 ubiquitin-protein ligase Mdm2 is a protein that in humans is encoded by the MDM2 gene. Mdm2 is an important negative regulator of the p53 tumor suppressor. Mdm2 protein functions both as an E3 ubiquitin ligase that recognizes the N-terminal trans-activation domain (TAD) of the p53 tumor suppressor and as an inhibitor of p53 transcriptional activation.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human MDM2 antibody. MDM2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MDM2 Antibody is added and binds to MDM2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MDM2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MDM2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cell Biology
Also known as
ACTFS;Double minute 2 protein;E3 ubiquitin-protein ligase Mdm2;hdm2;HDMX;LSKB;MDM2;Oncoprotein Mdm2;p53-binding protein Mdm2;RING-type E3 ubiquitin transferase Mdm2
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.33ng/L
Detection range
20-4500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
55,233 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5213Hu
Gene symbol
MDM2
Gene ID
4193
UniProt accession
Q00987
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

MDM2MDM2 proto-oncogene

View target guide

MDM2 identifies MDM2 proto-oncogene in Homo sapiens (Human). This target record connects NCBI Gene 4193, UniProtKB Q00987 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
4193
UniProtKB
Q00987
BT LAB
£240.93£205.00