Manufactured by:BT LAB
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Human E3 Ubiquitin-protein Ligase Chip, STUB1 ELISA Kit

Human E3 Ubiquitin-protein Ligase Chip, STUB1 ELISA Kit

SKU:
ABM216294
Manufacturer SKU
E5211Hu
Product type
ELISA Kits
TargetSTUB1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

STUB1 (STIP1 homology and U-Box containing protein 1) is a human gene that codes for the protein CHIP (C terminus of HSC70-Interacting Protein).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human STUB1 antibody. STUB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human STUB1 Antibody is added and binds to STUB1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated STUB1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human STUB1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
Antigen NY-CO-7;Carboxy terminus of Hsp70-interacting protein;CHIP;CLL-associated antigen KW-8;E3 ubiquitin-protein ligase CHIP;HSPABP2;NY-CO-7;RING-type E3 ubiquitin transferase CHIP;SCA48;SCAR16;STIP1 homology and U box-containing protein 1;STUB1;UBOX1;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
7.46ng/L
Detection range
15-3000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
34,856 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5211Hu
Gene symbol
STUB1
Gene ID
10273
UniProt accession
Q9UNE7
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

STUB1STIP1 homology and U-box containing protein 1

View target guide

STUB1 identifies STIP1 homology and U-box containing protein 1 in Homo sapiens (Human). This target record connects NCBI Gene 10273, UniProtKB Q9UNE7 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
10273
UniProtKB
Q9UNE7
BT LAB
£240.93£205.00