Human CCR4-not Transcription Complex Subunit 4, CNOT4 ELISA Kit

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Human CCR4-not Transcription Complex Subunit 4, CNOT4 ELISA Kit

Human CCR4-not Transcription Complex Subunit 4, CNOT4 ELISA Kit

SKU:
ABM216014
Manufacturer SKU
E5012Hu
Product type
ELISA Kits
TargetCNOT4
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

CCR4-NOT transcription complex, subunit 4 is a protein that in humans is encoded by the CNOT4 gene.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CNOT4 antibody. CNOT4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CNOT4 Antibody is added and binds to CNOT4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CNOT4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CNOT4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cell Biology
Also known as
CCR4-associated factor 4;CCR4-NOT transcription complex subunit 4;CLONE243;CNOT4;E3 ubiquitin-protein ligase CNOT4;NOT4;NOT4H;Potential transcriptional repressor NOT4Hp;RING-type E3 ubiquitin transferase CNOT4;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
7.15ng/L
Detection range
15-3000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
63,510 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5012Hu
Gene symbol
CNOT4
Gene ID
4850
UniProt accession
O95628
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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