Human Kruppel Like Factor 15, KLF15 ELISA Kit

BT LAB manufacturer product image

100%
Manufactured by:BT LAB
Save 15%RUO

Human Kruppel Like Factor 15, KLF15 ELISA Kit

Human Kruppel Like Factor 15, KLF15 ELISA Kit

SKU:
ABM215688
Manufacturer SKU
E4695Hu
Product type
ELISA Kits
TargetKLF15
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Transcriptional regulator that binds to the GA element of the CLCNKA promoter. Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart (By similarity). Is a repressor of CCN2 expression, involved in the control of cardiac fibrosis. It is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A and GATA4 (By similarity). Involved in podocyte differentiation (By similarity). Inhibits MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human KLF15 antibody. KLF15 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human KLF15 Antibody is added and binds to KLF15 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated KLF15 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human KLF15. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Epigenetics and Nuclear Signaling
Also known as
Kidney-enriched krueppel-like factor;KLF 15;KLF15;Krueppel-like factor 15
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.11ng/ml
Detection range
0.2-60ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
43,992 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E4695Hu
Gene symbol
KLF15
Gene ID
28999
UniProt accession
Q9UIH9
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00