Manufactured by:BT LAB
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Human T-cell-specific Surface Glycoprotein Cd28, CD28 ELISA Kit

Human T-cell-specific Surface Glycoprotein Cd28, CD28 ELISA Kit

SKU:
ABM215404
Manufacturer SKU
E4196hu
Product type
ELISA Kits
TargetCD28
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed: 8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed: 15067037).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CD28 antibody. CD28 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CD28 Antibody is added and binds to CD28 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD28 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CD28. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
CD 28;CD antigen CD28;CD28;T-cell-specific surface glycoprotein CD28;TP44
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.024ng/ml
Detection range
0.05-30ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
25,066 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E4196hu
Gene symbol
CD28
Gene ID
940
UniProt accession
P10747
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CD28CD28 molecule

View target guide

CD28 identifies CD28 molecule in Homo sapiens (Human). This target record connects NCBI Gene 940, UniProtKB P10747 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
940
UniProtKB
P10747
BT LAB
£240.93£205.00