Human Interferon-induced Protein with Tetratricopeptide Repeats 2, IFIT2 ELISA Kit

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Human Interferon-induced Protein with Tetratricopeptide Repeats 2, IFIT2 ELISA Kit

Human Interferon-induced Protein with Tetratricopeptide Repeats 2, IFIT2 ELISA Kit

SKU:
ABM215218
Manufacturer SKU
E3973Hu
Product type
ELISA Kits
TargetIFIT2
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human IFIT2 antibody. IFIT2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IFIT2 Antibody is added and binds to IFIT2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IFIT2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IFIT2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
cig42;G10P2;GARG-39;IFI54;IFI-54;IFI-54K;IFIT2;IFIT-2;Interferon-induced 54 kDa protein;Interferon-induced protein with tetratricopeptide repeats 2;ISG54;ISG-54;ISG-54 K;ISG-54K;P54
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
2.58ng/L
Detection range
5-2000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
54,632 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3973Hu
Gene symbol
IFIT2
Gene ID
3433
UniProt accession
P09913
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00