Manufactured by:BT LAB
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Human Urate Transporter 1, URAT1 ELISA Kit

Human Urate Transporter 1, URAT1 ELISA Kit

SKU:
ABM214702
Manufacturer SKU
E3340Hu
Product type
ELISA Kits
TargetSLC14A1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Urea Channel that facilitates transmembrane urea transport down a concentration gradient. A constriction of the transmembrane Channel functions as selectivity filter through which urea is expected to pass in dehydrated form. The rate of urea conduction is increased by hypotonic stress. Plays an important role in the kidney medulla collecting ducts, where it allows rapid equilibration between the lumen of the collecting ducts and the interstitium, and thereby prevents water loss driven by the high concentration of urea in the urine. Facilitates urea transport across erythrocyte membranes. May also play a role in transmembrane water transport, possibly by indirect means.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SLC14A1 antibody. SLC14A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SLC14A1 Antibody is added and binds to SLC14A1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SLC14A1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SLC14A1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Metabolism
Also known as
SLC14A1;Solute carrier family 14 member 1;Urea transporter 1;Urea transporter, erythrocyte
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.043ng/ml
Detection range
0.1-38ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
42,528 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3340Hu
Gene symbol
SLC14A1
Gene ID
6563
UniProt accession
Q13336
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00