Manufactured by:BT LAB
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Human Peroxiredoxin 2, PRDX2 ELISA Kit

Human Peroxiredoxin 2, PRDX2 ELISA Kit

SKU:
ABM214240
Manufacturer SKU
E2925Hu
Product type
ELISA Kits
TargetPRDX2
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PRDX2 antibody. PRDX2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PRDX2 Antibody is added and binds to PRDX2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PRDX2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PRDX2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cell Biology
Also known as
Natural killer cell-enhancing factor B;NKEF-B;Peroxiredoxin-2;PRDX 2;PRDX2;PRP;Thiol-specific antioxidant protein;Thioredoxin peroxidase 1;Thioredoxin-dependent peroxide reductase 1;Thioredoxin-dependent peroxiredoxin 2;TSA
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.58ng/L
Detection range
20-6000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
21,892 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2925Hu
Gene symbol
PRDX2
Gene ID
7001
UniProt accession
P32119
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

PRDX2peroxiredoxin 2

View target guide

PRDX2 identifies peroxiredoxin 2 in Homo sapiens (Human). This target record connects NCBI Gene 7001, UniProtKB P32119 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
7001
UniProtKB
P32119
BT LAB
£240.93£205.00