Human Gm2 Ganglioside Activator, GM2A ELISA Kit

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Human Gm2 Ganglioside Activator, GM2A ELISA Kit

Human Gm2 Ganglioside Activator, GM2A ELISA Kit

SKU:
ABM213392
Manufacturer SKU
E2504Hu
Product type
ELISA Kits
TargetGM2A
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity (By similarity). Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3 (By similarity). Has cholesterol transfer activity (PubMed:17552909).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human GM2A antibody. GM2A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GM2A Antibody is added and binds to GM2A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GM2A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GM2A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Tags & Cell Markers
Also known as
Cerebroside sulfate activator protein;Ganglioside GM2 activator;Ganglioside GM2 activator isoform short];GM2A;GM2-AP;SAP-3;Sphingolipid activator protein 3
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.052ng/ml
Detection range
0.1-40ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
20,838 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2504Hu
Gene symbol
GM2A
Gene ID
2760
UniProt accession
P17900
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00