Manufactured by:BT LAB
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Mouse Syndecan 4, SDC4 ELISA Kit

Mouse Syndecan 4, SDC4 ELISA Kit

SKU:
ABM213380
Manufacturer SKU
E2500Mo
Product type
ELISA Kits
TargetSDC4
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse SDC4 antibody. SDC4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SDC4 Antibody is added and binds to SDC4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SDC4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse SDC4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
Ryudocan core protein;SDC 4;SDC4;SYND4;Syndecan-4
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.56ng/L
Detection range
1-400ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
21,482 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2500Mo
Gene symbol
SDC4
Gene ID
20971
UniProt accession
O35988
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SDC4syndecan 4

View target guide

SDC4 identifies syndecan 4 in Homo sapiens (Human). This target record connects NCBI Gene 6385, UniProtKB P31431 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6385
UniProtKB
P31431
BT LAB
£240.93£205.00