Manufactured by:BT LAB
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Mouse Peroxiredoxin-1, PRDX1 ELISA Kit

Mouse Peroxiredoxin-1, PRDX1 ELISA Kit

SKU:
ABM212878
Manufacturer SKU
E2337Mo
Product type
ELISA Kits
TargetPRDX1
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2) (By similarity). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (PubMed: 19766572).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse PRDX1 antibody. PRDX1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse PRDX1 Antibody is added and binds to PRDX1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PRDX1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse PRDX1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cell Biology
Also known as
Macrophage 23 kDa stress protein;OSF-3;Osteoblast-specific factor 3;Peroxiredoxin-1;PRDX 1;PRDX1;Thioredoxin peroxidase 2;Thioredoxin-dependent peroxide reductase 2;Thioredoxin-dependent peroxiredoxin 1;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.056ng/mL
Detection range
0.1-40ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
22,176 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2337Mo
Gene symbol
PRDX1
Gene ID
18477
UniProt accession
P35700
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

PRDX1peroxiredoxin 1

View target guide

PRDX1 identifies peroxiredoxin 1 in Homo sapiens (Human). This target record connects NCBI Gene 5052, UniProtKB Q06830 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
5052
UniProtKB
Q06830
BT LAB
£240.93£205.00