Manufactured by:BT LAB
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Human Myosin Regulatory Light Polypeptide 9, MYL9 ELISA Kit

Human Myosin Regulatory Light Polypeptide 9, MYL9 ELISA Kit

SKU:
ABM212492
Manufacturer SKU
E2258Hu
Product type
ELISA Kits
TargetMYL9
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed: 11942626, PubMed: 2526655). In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human MYL9 antibody. MYL9 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MYL9 Antibody is added and binds to MYL9 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MYL9 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MYL9. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
20 kDa myosin light chain;LC20;MLC-2C;MYL 9;MYL9;Myosin regulatory light chain 2, smooth muscle isoform;Myosin regulatory light chain 9;Myosin regulatory light chain MRLC1;Myosin regulatory light polypeptide 9;Myosin RLC
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
7.74ng/L
Detection range
15-3000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
19,827 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2258Hu
Gene symbol
MYL9
Gene ID
10398
UniProt accession
P24844
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

MYL9myosin light chain 9

View target guide

MYL9 identifies myosin light chain 9 in Homo sapiens (Human). This target record connects NCBI Gene 10398, UniProtKB P24844 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
10398
UniProtKB
P24844
BT LAB
£240.93£205.00