Manufactured by:BT LAB
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Rat Protein S100-A1, S100A1 ELISA Kit

Rat Protein S100-A1, S100A1 ELISA Kit

SKU:
ABM212260
Manufacturer SKU
E2219Ra
Product type
ELISA Kits
TargetS100A1
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Probably acts as a Ca(2+) signal transducer (By similarity). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers a conformational change (PubMed: 16169012, PubMed: 18650434). This conformational change allows interaction of S1001A with specific target proteins, such as TPR-containing proteins, and the modulation of their activity (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat S100A1 antibody. S100A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat S100A1 Antibody is added and binds to S100A1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated S100A1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat S100A1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
Protein S100-A1;S100 calcium-binding protein A1;S-100 protein alpha chain;S-100 protein subunit alpha;S100A1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
1.57ng/mL
Detection range
3-900ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
10,560 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2219Ra
Gene symbol
S100A1
Gene ID
295214
UniProt accession
P35467
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

S100A1S100 calcium binding protein A1

View target guide

S100A1 identifies S100 calcium binding protein A1 in Homo sapiens (Human). This target record connects NCBI Gene 6271, UniProtKB P23297 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6271
UniProtKB
P23297
BT LAB
£240.93£205.00