Manufactured by:BT LAB
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Mouse Long-chain Fatty Acid Transport Protein 4, SLC27A4 ELISA Kit

Mouse Long-chain Fatty Acid Transport Protein 4, SLC27A4 ELISA Kit

SKU:
ABM212242
Manufacturer SKU
E2216Mo
Product type
ELISA Kits
TargetSLC27A4
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Long-chain fatty acid transport protein 4 is a protein that in humans is encoded by the SLC27A4 gene. This membrane protein is also called FATP4 or ACSVL5 (very long chain fatty acyl-CoA synthetase 5). The purified protein shows enzyme activity (EC 6.2.1.3), esterifying long and very long chain fatty acids with Coenzyme A. It is debated whether it is also a fatty acid transporter at the plasma membrane.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse SLC27A4 antibody. SLC27A4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SLC27A4 Antibody is added and binds to SLC27A4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SLC27A4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse SLC27A4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Neuroscience
Also known as
ACSVL4;Arachidonate--CoA ligase;BB144259;FAT;FATP4;FATP-4;Fatty acid transport protein 4;Long-chain fatty acid transport protein 4;Long-chain-fatty-acid--CoA ligase;SLC27A4;Solute carrier family 27 member 4;Very long-chain acyl-CoA synthetase 4
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.51ng/L
Detection range
20-4500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
72,319 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2216Mo
Gene symbol
Slc27a4
Gene ID
26569
UniProt accession
Q91VE0
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00