Mouse E-Selectin, SELE ELISA Kit

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Mouse E-Selectin, SELE ELISA Kit

Mouse E-Selectin, SELE ELISA Kit

SKU:
ABM211676
Manufacturer SKU
E2063Mo
Product type
ELISA Kits
TargetSELE
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1 (PubMed: 1375914). May have a role in capillary morphogenesis.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse SELE antibody. SELE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SELE Antibody is added and binds to SELE in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SELE antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse SELE. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
CD antigen CD62E;CD62 antigen-like family member E;ELAM-1;Endothelial leukocyte adhesion molecule 1;E-selectin;LECAM2;Leukocyte-endothelial cell adhesion molecule 2;SELE
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
14.94ng/L
Detection range
30-9000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
66,750 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2063Mo
Gene symbol
SELE
Gene ID
20339
UniProt accession
Q00690
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SELEselectin E

View target guide

SELE identifies selectin E in Homo sapiens (Human). This target record connects NCBI Gene 6401, UniProtKB P16581 and related ABMIUM catalogue products.

Biological function: SELE (E-selectin/CD62E) is an endothelial cell-surface adhesion glycoprotein that supports neutrophil adhesion to cytokine-activated endothelium, including through recognition of SELPLG/PSGL1.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6401
UniProtKB
P16581
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