Manufactured by:BT LAB
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Rat Fatty Acid Synthase, FAS ELISA Kit

Rat Fatty Acid Synthase, FAS ELISA Kit

SKU:
ABM211372
Manufacturer SKU
E1959Ra
Product type
ELISA Kits
TargetFASN
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat FASN antibody. FASN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat FASN Antibody is added and binds to FASN in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FASN antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat FASN. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Metabolism
Also known as
[Acyl-carrier-protein] S-malonyltransferase;3-hydroxyacyl-[acyl-carrier-protein] dehydratase;3-oxoacyl-[acyl-carrier-protein] reductase;3-oxoacyl-[acyl-carrier-protein] Synthase;Acyl-[acyl-carrier-protein] hydrolase;Enoyl-[acyl-carrier-protein] reductase;FASN;Fatty acid Synthase;Type I FAS [Includes: [Acyl-carrier-protein] S-acetyltransferase;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.27ng/mL
Detection range
0.5-200ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
272,650 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1959Ra
Gene symbol
FASN
Gene ID
50671
UniProt accession
P12785
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

FASNfatty acid synthase

View target guide

FASN identifies fatty acid synthase in Homo sapiens (Human). This target record connects NCBI Gene 2194, UniProtKB P49327 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
2194
UniProtKB
P49327
BT LAB
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