Manufactured by:BT LAB
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Rat Farnesyl Pyrophosphate Synthase, FDPS ELISA Kit

Rat Farnesyl Pyrophosphate Synthase, FDPS ELISA Kit

SKU:
ABM211370
Manufacturer SKU
E1957Ra
Product type
ELISA Kits
TargetFDPS
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat FDPS antibody. FDPS present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat FDPS Antibody is added and binds to FDPS in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FDPS antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat FDPS. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
(2E,6E-farnesyl diphosphate Synthase;Cholesterol-regulated 39 kDa protein;CR 39;Dimethylallyltranstransferase;Farnesyl diphosphate Synthase;Farnesyl pyrophosphate Synthase;FDPS;FPP Synthase;FPS;Geranyltranstransferase
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
7.16ng/L
Detection range
15-3000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
40,830 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1957Ra
Gene symbol
FDPS
Gene ID
83791
UniProt accession
P05369
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

FDPSfarnesyl diphosphate synthase

View target guide

FDPS identifies farnesyl diphosphate synthase in Homo sapiens (Human). This target record connects NCBI Gene 2224, UniProtKB P14324 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
2224
UniProtKB
P14324
BT LAB
£240.93£205.00