Manufactured by:BT LAB
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Rat Carboxypeptidase B, CPB1 ELISA Kit

Rat Carboxypeptidase B, CPB1 ELISA Kit

SKU:
ABM211172
Manufacturer SKU
E1894Ra
Product type
ELISA Kits
TargetCPB1
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Carboxypeptidase B (EC 3.4.17.2, protaminase, pancreatic carboxypeptidase B, tissue carboxypeptidase B, peptidyl-L-lysine [L-arginine]hydrolase) is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat CPB1 antibody. CPB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CPB1 Antibody is added and binds to CPB1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CPB1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CPB1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
Carboxypeptidase B;Cpb;CPB1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
3.12ng/mL
Detection range
7-1500ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
47,515 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1894Ra
Gene symbol
Cpb1
Gene ID
24271
UniProt accession
P19223
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CPB1carboxypeptidase B1

View target guide

CPB1 identifies carboxypeptidase B1 in Homo sapiens (Human). This target record connects NCBI Gene 1360, UniProtKB P15086 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
1360
UniProtKB
P15086
BT LAB
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