Manufactured by:BT LAB
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Rat Atp-binding Cassette Sub-family G Member 2, ABCG2 ELISA Kit

Rat Atp-binding Cassette Sub-family G Member 2, ABCG2 ELISA Kit

SKU:
ABM211118
Manufacturer SKU
E1878Ra
Product type
ELISA Kits
TargetABCG2
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

ATP-binding cassette super-family G member 2 is a protein that in humans is encoded by the ABCG2 gene. ABCG2 has also been designated as CDw338 (cluster of differentiation w338).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat ABCG2 antibody. ABCG2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ABCG2 Antibody is added and binds to ABCG2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ABCG2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat ABCG2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Tags & Cell Markers
Also known as
ABCG2;Adrenoleukodystrophy-related protein;ALDR;ATP-binding cassette sub-family D member 2
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.38ng/L
Detection range
20-4500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
83,369 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1878Ra
Gene symbol
Abcd2
Gene ID
84356
UniProt accession
Q9QY44
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ABCG2ATP binding cassette subfamily G member 2 (JR blood group)

View target guide

ATP binding cassette subfamily G member 2 (ABCG2), also called breast cancer resistance protein (BCRP) or CD338, is a broad-substrate ATP-dependent efflux transporter represented in the ABMIUM catalogue.

Biological function: ABCG2/BCRP is a broad-substrate ATP-dependent transporter that exports physiological compounds, dietary toxins and xenobiotics from cells. It contributes to multidrug resistance and also participates in transport of porphyrins, heme and urate.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
9429
UniProtKB
Q9UNQ0
BT LAB
£240.93£205.00