Manufactured by:BT LAB
Save 15%RUO

Rat Myosin Light Chain 3, MYL3 ELISA Kit

Rat Myosin Light Chain 3, MYL3 ELISA Kit

SKU:
ABM210924
Manufacturer SKU
E1812Ra
Product type
ELISA Kits
TargetMYL3
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Myosin essential light chain (ELC), ventricular/cardiac isoform is a protein that in humans is encoded by the MYL3 gene. This cardiac ventricular/slow skeletal ELC isoform is distinct from that expressed in fast skeletal muscle (MYL1) and cardiac atrial muscle (MYL4). Ventricular ELC is part of the myosin molecule and is important in modulating cardiac muscle contraction.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat MYL3 antibody. MYL3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MYL3 Antibody is added and binds to MYL3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MYL3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat MYL3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
MLC1SB;MLClV;MYL3;Mylc1v;Myosin alkali light chain 1, ventricular;Myosin light chain 1, slow-twitch muscle B/ventricular isoform;Myosin light chain 3;rVMLC1;Ventricular myosin light chain 1;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
6.99ng/L
Detection range
15-3000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
22,156 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1812Ra
Gene symbol
Myl3
Gene ID
24585
UniProt accession
P16409
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

MYL3myosin light chain 3

View target guide

MYL3 identifies myosin light chain 3 in Homo sapiens (Human). This target record connects NCBI Gene 4634, UniProtKB P08590 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
4634
UniProtKB
P08590
BT LAB
£240.93£205.00