Mouse Acrosin-binding Protein, ACRBP ELISA Kit

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Mouse Acrosin-binding Protein, ACRBP ELISA Kit

Mouse Acrosin-binding Protein, ACRBP ELISA Kit

SKU:
ABM210918
Manufacturer SKU
E1811Mo
Product type
ELISA Kits
TargetACRBP
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Acrosomal protein that maintains proacrosin (pro-ACR) as an enzymatically inactive zymogen in the acrosome. Involved also in the acrosome formation. Maintains pro-ACR as an enzymatically inactive zymogen in the acrosome until acrosomal exocytosis (PubMed:27303034). Partially contributes also to the assembly of acrosomal proteins to form an acrosomal granule (PubMed:27303034). Rodent specific isoform that participates in the formation of the acrosomal granule into the center of the acrosomal vesicle during early spermiogenesis (PubMed:27303034). In the fertilization process promotes ACR release from the acrosome during acrosomal exocytosis (PubMed:27303034).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse ACRBP antibody. ACRBP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ACRBP Antibody is added and binds to ACRBP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ACRBP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ACRBP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Tags & Cell Markers, Cancer
Also known as
ACRBP;Acrosin-binding protein;Acrosin-binding protein, 32 kDa form, mature form;Acrosin-binding protein, 60 kDa form;Acrosin-binding protein, mature form;ACRPB-C;OY-TES;OY-TES-1;Proacrosin-binding protein sp32;sp;sp32;Sp32]
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.026ng/mL
Detection range
0.05-20ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
61,141 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1811Mo
Gene symbol
Acrbp
Gene ID
54137
UniProt accession
Q3V140
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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