Manufactured by:BT LAB
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Rat Monocarboxylate Transporter-1, MCT-1 ELISA Kit

Rat Monocarboxylate Transporter-1, MCT-1 ELISA Kit

SKU:
ABM210676
Manufacturer SKU
E1673Ra
Product type
ELISA Kits
TargetSLC16A1
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat SLC16A1 antibody. SLC16A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SLC16A1 Antibody is added and binds to SLC16A1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SLC16A1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat SLC16A1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
MCT 1;Monocarboxylate transporter 1;SLC16A1;Solute carrier family 16 member 1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.058ng/ml
Detection range
0.1-35ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
53,238 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1673Ra
Gene symbol
SLC16A1
Gene ID
25027
UniProt accession
P53987
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SLC16A1solute carrier family 16 member 1

View target guide

SLC16A1 identifies solute carrier family 16 member 1 in Homo sapiens (Human). This target record connects NCBI Gene 6566, UniProtKB P53985 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6566
UniProtKB
P53985
BT LAB
£240.93£205.00