Rat Steroid 5-alpha-reductase 2, SRD5A2 ELISA Kit

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Rat Steroid 5-alpha-reductase 2, SRD5A2 ELISA Kit

Rat Steroid 5-alpha-reductase 2, SRD5A2 ELISA Kit

SKU:
ABM210546
Manufacturer SKU
E1585Ra
Product type
ELISA Kits
TargetSRD5A2
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Enables 3-oxo-5-alpha-steroid 4-dehydrogenase activity and amide binding activity. Involved in several processes, including androgen biosynthetic process; limbic system development; and reproductive structure development. Located in cell body fiber and neuronal cell body. Biomarker of diabetes mellitus and hyperprolactinemia. Human ortholog(s) of this gene implicated in hypospadias and prostate cancer. Orthologous to human SRD5A2 (steroid 5 alpha-reductase 2). [provided by Alliance of Genome Resources, Nov 2024]

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat SRD5A2 antibody. SRD5A2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SRD5A2 Antibody is added and binds to SRD5A2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SRD5A2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat SRD5A2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction, Cell Biology, Metabolism
Also known as
3-oxo-5-alpha-steroid 4-dehydrogenase 2;5 alpha-SR2;SR type 2;SRD5A2;Steroid 5-alpha-reductase 2
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.024ng/ml
Detection range
0.05-30ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
28,772 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1585Ra
Gene symbol
SRD5A2
Gene ID
64677
UniProt accession
P31214
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00