Manufactured by:BT LAB
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Human Receptor-type Tyrosine-protein Phosphatase T, PTPRT ELISA Kit

Human Receptor-type Tyrosine-protein Phosphatase T, PTPRT ELISA Kit

SKU:
ABM210530
Manufacturer SKU
E1579Hu
Product type
ELISA Kits
TargetPTPRT
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Receptor-type tyrosine-protein phosphatase T is an enzyme that in humans is encoded by the PTPRT gene. PTPRT is also known as PTPrho, PTPρ and human accelerated region 9. The human accelerated regions are 49 regions of the human genome that are conserved among vertebrates, but in humans show significant distinction from other vertebrates. This region may, therefore, have played a key role in differentiating humans from apes.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PTPRT antibody. PTPRT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PTPRT Antibody is added and binds to PTPRT in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PTPRT antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PTPRT. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
PTPRT;Receptor-type tyrosine-protein phosphatase rho;Receptor-type tyrosine-protein phosphatase T;RPTPrho;RPTP-rho;R-PTP-T
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.024ng/ml
Detection range
0.05-10ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
162,134 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1579Hu
Gene symbol
PTPRT
Gene ID
11122
UniProt accession
O14522
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00