Rat Endothelial Cell-specific Molecule 1, ESM-1 ELISA Kit

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Rat Endothelial Cell-specific Molecule 1, ESM-1 ELISA Kit

Rat Endothelial Cell-specific Molecule 1, ESM-1 ELISA Kit

SKU:
ABM209534
Manufacturer SKU
E0961Ra
Product type
ELISA Kits
TargetESM1
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat ESM1 antibody. ESM1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ESM1 Antibody is added and binds to ESM1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ESM1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat ESM1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Endothelial cell-specific molecule 1;ESM 1;ESM1;ESM-1;PG25
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.163ng/mL
Detection range
0.3-90ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
20,075 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0961Ra
Gene symbol
ESM1
Gene ID
64536
UniProt accession
P97682
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ESM1endothelial cell specific molecule 1

View target guide

ESM1 identifies endothelial cell specific molecule 1 in Homo sapiens (Human). This target record connects NCBI Gene 11082, UniProtKB Q9NQ30 and related ABMIUM catalogue products.

Biological function: Endothelial cell-specific molecule 1 (ESM1; endocan) is a secreted endothelial protein implicated in angiogenic sprouting and endothelial cell-leukocyte interactions. Its expression is regulated by inflammatory and angiogenic signals.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
11082
UniProtKB
Q9NQ30
Browse all ESM1 productsRead target context & evidence
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