Mouse Caspase 8, CASP8 ELISA Kit
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Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood (PubMed: 18455983, PubMed: 30361383, PubMed: 30381$458.00|$300.00, PubMed: 31511692, PubMed: 31748744). Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death (PubMed: 9654089, PubMed: 9837723, PubMed: 24813849, PubMed: 24813850). Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10 (By similarity). Binding to the adapter molecule FADD recruits it to either receptor TNFRSF6/FAS mediated or TNFRSF1A (PubMed: 29440439). The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation (By similarity). The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases (By similarity). Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC (By similarity). In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at 'Asp-325', which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response (PubMed: 31511692). Also able to initiate pyroptosis by mediating cleavage and activation of gasdermin-D (GSDMD): GSDMD cleavage promoting release of the N-terminal moiety (Gasdermin-D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis (PubMed: 30361383, PubMed: 30381$458.00|$300.00). Initiates pyroptosis following inactivation of MAP3K7/TAK1 (PubMed: 30361383, PubMed: 30381$458.00|$300.00). Also acts as a regulator of innate immunity by mediating cleavage and inactivation of N4BP1 downstream of TLR3 or TLR4, thereby promoting cytokine production (PubMed: 32971525). May participate in the Granzyme B (GZMB) cell death pathways (By similarity). Cleaves PARP1 (By similarity).
Fully tested in-house by ABMIUM, or supported by an advanced validation report from a partner reviewed by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Evidence status by application and model.
| Evidence | Serum | Plasma | Cell culture supernatants | Ascites | Tissue homogenates |
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This product is manufactured by BT LAB and sold through ABMIUM without relabelling.
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CASP8 identifies caspase 8 in Homo sapiens (Human). This target record connects NCBI Gene 841, UniProtKB Q14790 and related ABMIUM catalogue products.
Biological function: Caspase-8 (CASP8; FLICE/MACH) is an initiator cysteine protease activated in death-receptor signalling complexes. It cleaves downstream caspases to promote extrinsic apoptosis and also participates in regulated cell-death and inflammatory signalling.
