Manufactured by:BT LAB
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Human Α1-Antitrypsin, A1-AT ELISA Kit

Human Α1-Antitrypsin, A1-AT ELISA Kit

SKU:
ABM209194
Manufacturer SKU
E0753Hu
Product type
ELISA Kits
TargetSERPINA1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. [Short Peptide from AAT]: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SERPINA1 antibody. SERPINA1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SERPINA1 Antibody is added and binds to SERPINA1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SERPINA1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SERPINA1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular
Also known as
Alpha-1 protease inhibitor;Alpha-1-antiproteinase;Alpha-1-antitrypsin;Serpin A1;SERPINA 1;SERPINA1;Short Peptide from AAT;SPAAT
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.024mg/ml
Detection range
0.05-15mg/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
46,737 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0753Hu
Gene symbol
SERPINA1
Gene ID
5265
UniProt accession
P01009
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SERPINA1serpin family A member 1

View target guide

SERPINA1 identifies serpin family A member 1 in Homo sapiens (Human). This target record connects NCBI Gene 5265, UniProtKB P01009 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
5265
UniProtKB
P01009
BT LAB
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