Manufactured by:BT LAB
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Porcine Interleukin-7, IL7 ELISA Kit

Porcine Interleukin-7, IL7 ELISA Kit

SKU:
ABM208918
Manufacturer SKU
E0581Po
Product type
ELISA Kits
TargetIL7
ApplicationsELISA
Species / reactivityPorcine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine IL7 antibody. IL7 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine IL7 Antibody is added and binds to IL7 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL7 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine IL7. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
IL 7;IL7;IL-7;Interleukin-7
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.56ng/L
Detection range
1-400ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
20,161 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0581Po
Gene symbol
IL7
Gene ID
397253
UniProt accession
Q9N2G6
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IL7interleukin 7

View target guide

IL7 identifies interleukin 7 in Homo sapiens (Human). This target record connects NCBI Gene 3574, UniProtKB P13232 and related ABMIUM catalogue products.

Biological function: Interleukin 7 (IL7; IL-7) is a secreted cytokine with central roles in lymphoid-cell development, survival and homeostasis, particularly in T-cell development and maintenance. It signals through a receptor complex containing IL7R and the common gamma chain.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3574
UniProtKB
P13232
BT LAB
£240.93£205.00