Manufactured by:BT LAB
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Porcine Interferon Alpha, Beta Receptor 1, IFNAR1 ELISA Kit

Porcine Interferon Alpha, Beta Receptor 1, IFNAR1 ELISA Kit

SKU:
ABM208908
Manufacturer SKU
E0576Po
Product type
ELISA Kits
TargetIFNAR1
ApplicationsELISA
Species / reactivityPorcine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Component of the receptor for type I interferons, including interferons alpha, IFNB1 and IFNW1. Functions in general as heterodimer with IFNAR2. Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves (By similarity). Can form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine IFNAR1 antibody. IFNAR1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine IFNAR1 Antibody is added and binds to IFNAR1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IFNAR1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine IFNAR1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
IFN-alpha/beta receptor 1;IFNAR 1;IFNAR1;IFN-R-1;Interferon alpha/beta receptor 1;Type I interferon receptor 1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.055ng/mL
Detection range
0.1-40ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
63,221 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0576Po
Gene symbol
IFNAR1
Gene ID
396658
UniProt accession
Q764M8
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IFNAR1interferon alpha and beta receptor subunit 1

View target guide

IFNAR1 identifies interferon alpha and beta receptor subunit 1 in Homo sapiens (Human). This target record connects NCBI Gene 3454, UniProtKB P17181 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3454
UniProtKB
P17181
BT LAB
£240.93£205.00